WebThe Biomath Calculators provide a range of functions essential to molecular biology experiments, including nucleic acid and protein conversions, melting temperature, … WebLigation Calculator. This tool will calculate the mass of insert required at several molar insert:vector ratios in the range needed for typical ligation reactions. Ligation. Tutorials. Insert DNA length. Vector DNA length. Vector DNA mass. Required insert DNA mass. --- …
Cloning with pGEM®-T Vectors: Ligation - Promega Terminal
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Promega Biomath Calculators on the App Store
WebJan 8, 2024 · One are the simplest methods available cloning blunt-ended DNA fragmenting including PCR products is T-vector cloning, such as with pGEM®-T or pGEM®-T Easy Vector Our.This method takes advantage of the “A” overhang added by a PCR enzyme like Taq DNA Polymerase.T vectors are linearized plasmids the have be address the add 3′ T … WebJan 8, 2024 · One are the simplest methods available cloning blunt-ended DNA fragmenting including PCR products is T-vector cloning, such as with pGEM®-T or pGEM®-T Easy … Web- Easily calculate Molarity, Total Solution Volume, Solute Mass, or Molecular Weight. - Simply input three of the four variables and the fourth is calculated automatically. - The units can be changed for Total Solution Volume, Solute Mass, or Molecular Weight. - In-App purchase available to create a… how much is pff worth