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Orbitally shaken

Orbitally shaken bioreactors are widely used for small-scale screening and process optimization. Complex mechanical and electronic parts such as the shaker drive, the power train, and the control unit are integrated in the shaker and, therefore, separated from the reactor vessel. See more The well-defined and homogeneous liquid distribution during shaking is a key benefit of orbitally shaken single-use reactors. Reproducible flow … See more The applicability of orbitally shaken single-use reactors for aerobic bioprocesses highly depends on their potential to deliver a sufficient amount of oxygen to the cells. The first quantitative characterization of the oxygen transfer in … See more The first detailed characterization of the mixing performance in cylindrical orbitally shaken bioreactors with volumes ranging from 2 to 1,500 L was reported by Tissot et al. [29]. A calorimetric method was used to determine mixing … See more WebSep 1, 2014 · Study of the phase-resolved flow characteristics allowed to built a Re-Fr map, where four quadrants associated to different flow regimes are identified: in-phase toroidal vortex (low Fr and high Re), out-of-phase precessional vortex (high Fr and high Re), in-phase single vortex (low Fr and low Re), out-of-phase counter-rotating toroidal vortex …

Disposable bioreactors: the current state-of-the-art and …

WebJun 15, 2012 · The volumetric power input of the 200 L and 2000 L orbitally shaken bioreactors (Kühner AG, Birsfelden, Switzerland) was measured applying a slightly modified temperature method. The temperature method is based on the fact that the volume specific heat energy per time produced by the friction of the liquid on the walls of the bioreactor is ... WebJul 15, 2013 · Orbitally shaken bioreactors (OSRs) support the efficient cultivation of mammalian cells in suspension at various scales of operation. Here we introduce a disposable culture vessel with a ventilated cap having a nominal volume of 600 mL (TubeSpin ® bioreactor 600) for medium-scale cell cultivation. It has a conical bottom … cinemark card balance check https://keonna.net

Orbitally shaken single-use bioreactors. Semantic Scholar

WebAn Erlenmeyer flask, also known as a conical flask (British English) or a titration flask, is a type of laboratory flask which features a flat bottom, a conical body, and a cylindrical neck. It is named after the German chemist … WebNext Day Science has a wide variety of orbital shakers for sale, ranging from large to compact, and in varying speeds and capacities. Digital orbital shakers are available as … WebMar 24, 2024 · We present a new theoretical model describing gravity–capillary waves in orbitally shaken cylindrical containers. Our model can account for both one-layer free … diabetic supply new bern

Determination of a scale-up factor from mixing time studies in ...

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Orbitally shaken

Scalable Production of Aav Vectors In Orbitally Shaken Hek293 Cells

WebMar 30, 2024 · Orbital shakers are simple devices that provide mixing, aeration, and shear stress at multiple scales and high throughput. For this reason, they are extensively used in a wide range of applications from protein production to … WebJul 30, 2009 · Driven by the commercial success of recombinant biopharmaceuticals, there is an increasing demand for novel, cost-effective mammalian cell culture bioreactor systems for the production of biologicals requiring mammalian protein processing. Recently, orbitally shaken bioreactors at scales from a few mLs to 2'000-L have been explored for the …

Orbitally shaken

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WebMar 13, 2024 · Mode decomposition and Lagrangian structures of the flow dynamics in orbitally shaken bioreactors. Physics of Fluids , 2024; 30 (3): 033603 DOI: 10.1063/1.5016305 Cite This Page : WebThe shaker (25 mL–6 L) was the most widely used orbitally shaken bioreactor (OSB) type, providing a cost-effective system that is effective for a broad range of applications …

WebMar 1, 2024 · Orbitally shaken bioreactors (OSRs) is one of important bioreactors for mammalian cells cultivation in suspension, especially for the screening of valuable … WebJan 22, 2010 · An overview of the disposable bioreactors that are currently commercially available is provided, and the domination of wave-mixed, orbitally shaken, and, in particular, stirred disposable bioreactors in animal cell-derived productions at …

WebThe Boekel Scientific Orbital and Reciprocating Shaking Water Bath is designed for a wide range of temperature-controlled shaking protocols, such as Northern and Southern … WebJan 15, 2014 · At laboratory scale, cells are usually grown in low shear devices, with orbitally shaken bioreactors (OSRs) being largely employed in the early stages of bioprocess …

WebAug 19, 2024 · The orbitally shaken bioreactors (OSRs) were shaken on a platform connected to a shaker motor. The distance between the attachment point of the platform and the center axis of the shaker motor represents half the shaking diameter (= displacement radius). The OSRs contained deionized water maintained at 37 °C.

WebJun 14, 2024 · Cells were orbitally shaken at 180 rpm at 37°C with 5% CO 2 and 85% humidity in ISF-4-W incubators (Kühner, Birsfelden, Switzerland). Cells were seeded at 0.5 … cinemark canoas filmesWebThis study investigated the efficacy of a novel correlation of power input, energy dissipation rate and mixing time as a potential route to identify the orbitally shaken bioreactor (OSB) system. The Buckingham’s π-theorem was used to designate and transform dimensionless Newton numbers with five relevant power input variables. These variables … cinemark carnation cinema 5 showtimesWebShaken bioreactors were comprehensively utilized in the early stages of bioprocess development and microscale plates (24-, 48-, 96- or 384-orifice discs) facilitated a significant number of experiments. The shaker (25 mL–6 L) was the most widely used orbitally shaken bioreactor (OSB) type, providing a diabetic supply of the midlandsWebOrbitally shaken bioreactors (OSBs) utilize the same hydrodynamic principles that define the success of smaller-scale cultur … Increasing the cultivation volume from small to large … diabetic supply onlineWebThe SB10-X is an orbital shaken benchtop bioreactor system for the cultivation of human, mammalian and plant cells in a single-use bag. It has a working volume from 4 litres up to … diabetic supply omahaWebOrbitally shaken cylindrical bioreactors [OrbShake bioreactors (OSRs)] without an impeller or sparger are increasingly being used for the suspension cultivation of mammalian cells. Among small volume OSRs, 50-mL tubes with a ventilated cap (OSR50), originally derived from standard laboratory centrifuge tubes with a conical bottom, have found ... diabetic supply organizer blackWebNov 20, 2024 · Disposable orbitally shaken bioreactors have been widely used for mammalian cell culture in suspension. Three kinds of baffle structures: vertical baffle, inclined baffle and horizontal baffle were designed in this work. The flow fields of the shaking bioreactor with different baffle structures were … cinemark carefree circle and imax top gun