Rna cleaving dnazyme
WebOct 26, 2007 · SARS-CoV total RNA was isolated, extracted from the SARS-CoV-WHU strain and converted into cDNA. We designed a RNA-cleaving 10–23 DNAzyme targeting at the loop region of the 5′UTR of SARS-CoV. The designed DNAzyme, Dz-104, and its mutant version, Dz-104 (mut), as a control consist of 9 + 9 arm sequences with a 10–23 catalytic … WebFeb 19, 2013 · Modified 10–23 DNAzyme was found to be quite an efficient RNA-cleaving DNAzyme, with a k obs of 0.018 min −1 (Supplementary Fig. S1), which was relatively …
Rna cleaving dnazyme
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WebApr 7, 2024 · Monitoring the expression level of the PD-L1 protein on exosomes (exosomal PD-L1) is promising for both tumor diagnosis and tumor immunotherapy. Here, we established an aptamer-bivalent-cholesterol-anchor assembly of DNAzyme (ABCzyme) analytical strategy that can directly detect exosomal PD-L1 with a minimum lower limit of … WebSeveral representative RNA-cleaving DNAzymes and their metal ion cofactors are summarized in Figure 1.8. The 39E DNAzyme was isolated in the presence of UO22+ and it has over 1-million-fold higher selectivity for UO22+ over other metal ions
In addition to RNAs, 8–17 DNAzyme can also cleave DNA substrates with a single ribonucleotide at the cleavage site (Fig. 1b). To unravel the catalytic mechanism of the DNAzyme, we carried out crystallographic studies of 8–17 DNAzyme. Via screening various sequences, we fortunately obtained the crystals … See more Among the three structures, DNAzyme-Pb2+ complex diffracted to the highest resolution (2.55 Å) and refinement resulted in a well-defined electron density for all … See more In all the DNAzyme/substrate complex structures, the substrate cleavage site residues G+1 and G−1 are kinked and pair with the nucleotides T1 and A14 of the … See more The conformations of the DNAzyme-Pb2+ and DNAzyme/native DNA complex without Pb2+ are virtually identical; in both structures, the N1 atom of G13 forms one … See more Very fortunately, an in-line arrangement of the nucleophile (O2′ atom of G−1), the electrophilic center (P atom of 3′-phosphate of G−1), and the leaving … See more WebSep 19, 2024 · Our laboratory has a keen interest in searching for diverse DNAzymes capable of cleaving RNA-containing substrates, determining their sequence requirements and structural properties, and examining their potential as biosensors. This Account begins with the description of an accidental discovery on the sequence adaptability of a small …
WebConférencier : Scott Smith a obtenu un bac (1994) et un doctorat (1999) en géochimie à l’université McMaster. Il a affectué un post-doctorat au département de géologie de l’Université de Toronto avant d’intégrer l’Université Wilfrid Laurier (Waterloo, Ontario) en … WebDNAzyme motors are widely used for the sensitive detection of intracellular miRNAs due to their excellent signal response. Generally, the addition of exogenous mental ions to DNAzyme motors is crucial for the efficient operation of the system. Moreover, the position of the DNAzyme relative to the su …
WebApr 27, 2010 · Moreover, the function of 8–17 has been regarded with special importance because it can catalyze sequence-specific cleavage of RNA, a reaction that has broad implications in biotechnology and biomedical fields. In this review, we explore the creation, characterization, and application of the 8–17 RNA-cleaving DNAzyme.
WebApr 27, 2015 · Here, we report the in vitro selection of the first (to our knowledge) Na +-specific, RNA-cleaving deoxyribozyme (DNAzyme) with a fast catalytic rate [observed rate constant (k obs) ∼0.1 min −1], and the transformation of this DNAzyme into a fluorescent sensor for Na + by labeling the enzyme strand with a quencher at the 3′ end, and the ... rooney nyrWebNov 16, 2024 · (A) A representative RNA-cleaving DNAzyme; (B) The common RNA cleavage reaction catalyzed by a RNA-cleaving DNAzyme, resulting in two short nucleic acid … rooney no hairWebOct 15, 2003 · Abstract. '10-23' DNAzymes can be used to cleave any target RNA in a sequence-specific manner. For applications in vivo, they have to be stabilised against … rooney novel